High Efficiency of Stable
Transformation of a Dendrobium Orchid Via
Microprojectile Bombardment
Usa Ketsuwan1, Jittapan Piluk2,
Salak Pasiri3, and Pattana Srifah Huehne1,4
Salak Pasiri3, and Pattana Srifah Huehne1,4
Transient gus
expression was used to evaluate efficiency of promoter for genetic transformation
of Dendrobium Jaquelyn Thomas via microprojectile bombardment. Among three
plant promoters driving gus gene, actin promoter of rice gave the highest
expression on the orchid flower while the other CaMV35S and Ubiquitin
promoter gave a few GUS blue focies. The optimize parameters of bombardment
for stable transformation in orchid plbs were also investigated using
gfp (pMNG1005) as reporter gene. The result was similar to a standard
parameter with He presure 1,100 psi at the taget distance 9 cm. Further
study on the critical point to achieve for orchid stable transformation
was performed and found that the most success at a high efficiency of
transformation depending on the stage of growth of plbs and their background
on baic tissue culture performance.
1 Department of Genetics and
2Department of Horticulture, Kasetsart University, Bangkok 10900, Thailand;
3KURDI, Kasetsart University, Bangkok 10900, Thailand;
4 Laboratory of Biotechnology, Chulabhorn Research Institute, Lak Si,
Bangkok 10210, Thailand