SEQUENCE ANALYSIS OF CHLOROPLAST
16SrDNA-RPS12/7 AND ITS
SPACER OF DENDROBIUM ORCHIDS
Benjawan Lertwiriyawong1, Panapa Saksoong2
and Pattana Srifah Huehne1,2
The 16S rDNA-rps12/7
and its spacer region of the chloroplast DNA were isolated from two orchids,
Dendrobium Jaquelyne Thomas and Den. Ear Sakul. The amplified fragments
of 3,782 bp from total orchid DNA extraction were sequenced and evaluated.
We found that 16S rDNA-rps12/7 nucleotide sequences of two orchids were
identical. Surprisingly, the alignment of their nucleotides with other
published sequences show that orchid chloroplast 16S rDNA and its inter
spacer between 16SrDNA and rps12/7 are similar to dicots, Amborella trichopoda
and Nicotiana tabacum but differ from other monocots including Triticum
aestivum, Oryza sativa and Zea mays. According to the high conserve sequence
in 16S rDNA-rps12/7 region, it is possible to integrate this region into
vector cassette of tobacco plastid transformation vector and apply as
a vector for orchid transformation.
1 Department of Genetics, Kasetsart University, Bangkok
10900, Thailand;
2 Laboratory of Biotechnology, Chulabhorn Research Institute, Lak Si,
Bangkok10210, Thailand.